eea1 antibody Search Results


a32733  (R&D Systems)
93
R&D Systems a32733
A32733, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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eea1  (Novus Biologicals)
92
Novus Biologicals eea1
Figure 8—TLR4-Ab remains colocalized with <t>EEA1</t> and sequesters TLR4/MD2 inside the endosome for at least 24 h. BMDMs were pulsed with LPS (100 ng) or TLR4-Ab (UT18 100 ng) for 5 min, washed, and then fixed at the noted time points. Cells were stained (CD11b, TLR4/ UT18, EEA1) for imaging flow cytometry, and 5,000 events (gated on CD11b1 cells) were collected for each cell population. A and B: Rep- resentative images for LPS- and TLR4-Ab–stimulated BMDMs are shown for each time point. C: Internalization analysis generated using IDEAS software provides a mean internalization erode score using CD11b as the surface marker and TLR4/UT18 as the internalization probe. TLR4 internalization scores >1.0 indicate internalization of TLR4. P values calculated in GraphPad Prism. *P < 0.05, ****P < 0.0001. D: Bright detail similarity (median) measures pixel-by-pixel correlation between TLR4 and EEA1 in each image, effectively measur- ing colocalization of the two probes at each denoted time point. Bright detail similarity >1.0 indicates colocalization of TLR4 and EEA1. *P 5 0.014, **P 5 0.0067. The quantification of C and D represent data collected from three independent experiments.
Eea1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/eea1/product/Novus Biologicals
Average 92 stars, based on 1 article reviews
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rabbit polyclonal anti eea1  (Cell Signaling Technology Inc)
97
Cell Signaling Technology Inc rabbit polyclonal anti eea1
Figure 8—TLR4-Ab remains colocalized with <t>EEA1</t> and sequesters TLR4/MD2 inside the endosome for at least 24 h. BMDMs were pulsed with LPS (100 ng) or TLR4-Ab (UT18 100 ng) for 5 min, washed, and then fixed at the noted time points. Cells were stained (CD11b, TLR4/ UT18, EEA1) for imaging flow cytometry, and 5,000 events (gated on CD11b1 cells) were collected for each cell population. A and B: Rep- resentative images for LPS- and TLR4-Ab–stimulated BMDMs are shown for each time point. C: Internalization analysis generated using IDEAS software provides a mean internalization erode score using CD11b as the surface marker and TLR4/UT18 as the internalization probe. TLR4 internalization scores >1.0 indicate internalization of TLR4. P values calculated in GraphPad Prism. *P < 0.05, ****P < 0.0001. D: Bright detail similarity (median) measures pixel-by-pixel correlation between TLR4 and EEA1 in each image, effectively measur- ing colocalization of the two probes at each denoted time point. Bright detail similarity >1.0 indicates colocalization of TLR4 and EEA1. *P 5 0.014, **P 5 0.0067. The quantification of C and D represent data collected from three independent experiments.
Rabbit Polyclonal Anti Eea1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1 ig  (Proteintech)
95
Proteintech 1 ig
Figure 8—TLR4-Ab remains colocalized with <t>EEA1</t> and sequesters TLR4/MD2 inside the endosome for at least 24 h. BMDMs were pulsed with LPS (100 ng) or TLR4-Ab (UT18 100 ng) for 5 min, washed, and then fixed at the noted time points. Cells were stained (CD11b, TLR4/ UT18, EEA1) for imaging flow cytometry, and 5,000 events (gated on CD11b1 cells) were collected for each cell population. A and B: Rep- resentative images for LPS- and TLR4-Ab–stimulated BMDMs are shown for each time point. C: Internalization analysis generated using IDEAS software provides a mean internalization erode score using CD11b as the surface marker and TLR4/UT18 as the internalization probe. TLR4 internalization scores >1.0 indicate internalization of TLR4. P values calculated in GraphPad Prism. *P < 0.05, ****P < 0.0001. D: Bright detail similarity (median) measures pixel-by-pixel correlation between TLR4 and EEA1 in each image, effectively measur- ing colocalization of the two probes at each denoted time point. Bright detail similarity >1.0 indicates colocalization of TLR4 and EEA1. *P 5 0.014, **P 5 0.0067. The quantification of C and D represent data collected from three independent experiments.
1 Ig, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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eea1  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology eea1
Figure 8—TLR4-Ab remains colocalized with <t>EEA1</t> and sequesters TLR4/MD2 inside the endosome for at least 24 h. BMDMs were pulsed with LPS (100 ng) or TLR4-Ab (UT18 100 ng) for 5 min, washed, and then fixed at the noted time points. Cells were stained (CD11b, TLR4/ UT18, EEA1) for imaging flow cytometry, and 5,000 events (gated on CD11b1 cells) were collected for each cell population. A and B: Rep- resentative images for LPS- and TLR4-Ab–stimulated BMDMs are shown for each time point. C: Internalization analysis generated using IDEAS software provides a mean internalization erode score using CD11b as the surface marker and TLR4/UT18 as the internalization probe. TLR4 internalization scores >1.0 indicate internalization of TLR4. P values calculated in GraphPad Prism. *P < 0.05, ****P < 0.0001. D: Bright detail similarity (median) measures pixel-by-pixel correlation between TLR4 and EEA1 in each image, effectively measur- ing colocalization of the two probes at each denoted time point. Bright detail similarity >1.0 indicates colocalization of TLR4 and EEA1. *P 5 0.014, **P 5 0.0067. The quantification of C and D represent data collected from three independent experiments.
Eea1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/eea1/product/Santa Cruz Biotechnology
Average 96 stars, based on 1 article reviews
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anti β 1 integrin mab13 antibody  (Biorbyt)
92
Biorbyt anti β 1 integrin mab13 antibody
Figure 8—TLR4-Ab remains colocalized with <t>EEA1</t> and sequesters TLR4/MD2 inside the endosome for at least 24 h. BMDMs were pulsed with LPS (100 ng) or TLR4-Ab (UT18 100 ng) for 5 min, washed, and then fixed at the noted time points. Cells were stained (CD11b, TLR4/ UT18, EEA1) for imaging flow cytometry, and 5,000 events (gated on CD11b1 cells) were collected for each cell population. A and B: Rep- resentative images for LPS- and TLR4-Ab–stimulated BMDMs are shown for each time point. C: Internalization analysis generated using IDEAS software provides a mean internalization erode score using CD11b as the surface marker and TLR4/UT18 as the internalization probe. TLR4 internalization scores >1.0 indicate internalization of TLR4. P values calculated in GraphPad Prism. *P < 0.05, ****P < 0.0001. D: Bright detail similarity (median) measures pixel-by-pixel correlation between TLR4 and EEA1 in each image, effectively measur- ing colocalization of the two probes at each denoted time point. Bright detail similarity >1.0 indicates colocalization of TLR4 and EEA1. *P 5 0.014, **P 5 0.0067. The quantification of C and D represent data collected from three independent experiments.
Anti β 1 Integrin Mab13 Antibody, supplied by Biorbyt, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti β 1 integrin mab13 antibody/product/Biorbyt
Average 92 stars, based on 1 article reviews
anti β 1 integrin mab13 antibody - by Bioz Stars, 2026-03
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mouse anti eea1  (R&D Systems)
93
R&D Systems mouse anti eea1
Figure 8—TLR4-Ab remains colocalized with <t>EEA1</t> and sequesters TLR4/MD2 inside the endosome for at least 24 h. BMDMs were pulsed with LPS (100 ng) or TLR4-Ab (UT18 100 ng) for 5 min, washed, and then fixed at the noted time points. Cells were stained (CD11b, TLR4/ UT18, EEA1) for imaging flow cytometry, and 5,000 events (gated on CD11b1 cells) were collected for each cell population. A and B: Rep- resentative images for LPS- and TLR4-Ab–stimulated BMDMs are shown for each time point. C: Internalization analysis generated using IDEAS software provides a mean internalization erode score using CD11b as the surface marker and TLR4/UT18 as the internalization probe. TLR4 internalization scores >1.0 indicate internalization of TLR4. P values calculated in GraphPad Prism. *P < 0.05, ****P < 0.0001. D: Bright detail similarity (median) measures pixel-by-pixel correlation between TLR4 and EEA1 in each image, effectively measur- ing colocalization of the two probes at each denoted time point. Bright detail similarity >1.0 indicates colocalization of TLR4 and EEA1. *P 5 0.014, **P 5 0.0067. The quantification of C and D represent data collected from three independent experiments.
Mouse Anti Eea1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti eea1/product/R&D Systems
Average 93 stars, based on 1 article reviews
mouse anti eea1 - by Bioz Stars, 2026-03
93/100 stars
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anti eea1  (Bethyl)
93
Bethyl anti eea1
Figure 8—TLR4-Ab remains colocalized with <t>EEA1</t> and sequesters TLR4/MD2 inside the endosome for at least 24 h. BMDMs were pulsed with LPS (100 ng) or TLR4-Ab (UT18 100 ng) for 5 min, washed, and then fixed at the noted time points. Cells were stained (CD11b, TLR4/ UT18, EEA1) for imaging flow cytometry, and 5,000 events (gated on CD11b1 cells) were collected for each cell population. A and B: Rep- resentative images for LPS- and TLR4-Ab–stimulated BMDMs are shown for each time point. C: Internalization analysis generated using IDEAS software provides a mean internalization erode score using CD11b as the surface marker and TLR4/UT18 as the internalization probe. TLR4 internalization scores >1.0 indicate internalization of TLR4. P values calculated in GraphPad Prism. *P < 0.05, ****P < 0.0001. D: Bright detail similarity (median) measures pixel-by-pixel correlation between TLR4 and EEA1 in each image, effectively measur- ing colocalization of the two probes at each denoted time point. Bright detail similarity >1.0 indicates colocalization of TLR4 and EEA1. *P 5 0.014, **P 5 0.0067. The quantification of C and D represent data collected from three independent experiments.
Anti Eea1, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti eea1/product/Bethyl
Average 93 stars, based on 1 article reviews
anti eea1 - by Bioz Stars, 2026-03
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anti eea1  (R&D Systems)
93
R&D Systems anti eea1
Figure 8—TLR4-Ab remains colocalized with <t>EEA1</t> and sequesters TLR4/MD2 inside the endosome for at least 24 h. BMDMs were pulsed with LPS (100 ng) or TLR4-Ab (UT18 100 ng) for 5 min, washed, and then fixed at the noted time points. Cells were stained (CD11b, TLR4/ UT18, EEA1) for imaging flow cytometry, and 5,000 events (gated on CD11b1 cells) were collected for each cell population. A and B: Rep- resentative images for LPS- and TLR4-Ab–stimulated BMDMs are shown for each time point. C: Internalization analysis generated using IDEAS software provides a mean internalization erode score using CD11b as the surface marker and TLR4/UT18 as the internalization probe. TLR4 internalization scores >1.0 indicate internalization of TLR4. P values calculated in GraphPad Prism. *P < 0.05, ****P < 0.0001. D: Bright detail similarity (median) measures pixel-by-pixel correlation between TLR4 and EEA1 in each image, effectively measur- ing colocalization of the two probes at each denoted time point. Bright detail similarity >1.0 indicates colocalization of TLR4 and EEA1. *P 5 0.014, **P 5 0.0067. The quantification of C and D represent data collected from three independent experiments.
Anti Eea1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti eea1/product/R&D Systems
Average 93 stars, based on 1 article reviews
anti eea1 - by Bioz Stars, 2026-03
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sheep  (R&D Systems)
93
R&D Systems sheep
Figure 8—TLR4-Ab remains colocalized with <t>EEA1</t> and sequesters TLR4/MD2 inside the endosome for at least 24 h. BMDMs were pulsed with LPS (100 ng) or TLR4-Ab (UT18 100 ng) for 5 min, washed, and then fixed at the noted time points. Cells were stained (CD11b, TLR4/ UT18, EEA1) for imaging flow cytometry, and 5,000 events (gated on CD11b1 cells) were collected for each cell population. A and B: Rep- resentative images for LPS- and TLR4-Ab–stimulated BMDMs are shown for each time point. C: Internalization analysis generated using IDEAS software provides a mean internalization erode score using CD11b as the surface marker and TLR4/UT18 as the internalization probe. TLR4 internalization scores >1.0 indicate internalization of TLR4. P values calculated in GraphPad Prism. *P < 0.05, ****P < 0.0001. D: Bright detail similarity (median) measures pixel-by-pixel correlation between TLR4 and EEA1 in each image, effectively measur- ing colocalization of the two probes at each denoted time point. Bright detail similarity >1.0 indicates colocalization of TLR4 and EEA1. *P 5 0.014, **P 5 0.0067. The quantification of C and D represent data collected from three independent experiments.
Sheep, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sheep/product/R&D Systems
Average 93 stars, based on 1 article reviews
sheep - by Bioz Stars, 2026-03
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anti eea1  (Cusabio)
92
Cusabio anti eea1
Figure 8—TLR4-Ab remains colocalized with <t>EEA1</t> and sequesters TLR4/MD2 inside the endosome for at least 24 h. BMDMs were pulsed with LPS (100 ng) or TLR4-Ab (UT18 100 ng) for 5 min, washed, and then fixed at the noted time points. Cells were stained (CD11b, TLR4/ UT18, EEA1) for imaging flow cytometry, and 5,000 events (gated on CD11b1 cells) were collected for each cell population. A and B: Rep- resentative images for LPS- and TLR4-Ab–stimulated BMDMs are shown for each time point. C: Internalization analysis generated using IDEAS software provides a mean internalization erode score using CD11b as the surface marker and TLR4/UT18 as the internalization probe. TLR4 internalization scores >1.0 indicate internalization of TLR4. P values calculated in GraphPad Prism. *P < 0.05, ****P < 0.0001. D: Bright detail similarity (median) measures pixel-by-pixel correlation between TLR4 and EEA1 in each image, effectively measur- ing colocalization of the two probes at each denoted time point. Bright detail similarity >1.0 indicates colocalization of TLR4 and EEA1. *P 5 0.014, **P 5 0.0067. The quantification of C and D represent data collected from three independent experiments.
Anti Eea1, supplied by Cusabio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti eea1/product/Cusabio
Average 92 stars, based on 1 article reviews
anti eea1 - by Bioz Stars, 2026-03
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ab0006  (OriGene)
94
OriGene ab0006
Figure 8—TLR4-Ab remains colocalized with <t>EEA1</t> and sequesters TLR4/MD2 inside the endosome for at least 24 h. BMDMs were pulsed with LPS (100 ng) or TLR4-Ab (UT18 100 ng) for 5 min, washed, and then fixed at the noted time points. Cells were stained (CD11b, TLR4/ UT18, EEA1) for imaging flow cytometry, and 5,000 events (gated on CD11b1 cells) were collected for each cell population. A and B: Rep- resentative images for LPS- and TLR4-Ab–stimulated BMDMs are shown for each time point. C: Internalization analysis generated using IDEAS software provides a mean internalization erode score using CD11b as the surface marker and TLR4/UT18 as the internalization probe. TLR4 internalization scores >1.0 indicate internalization of TLR4. P values calculated in GraphPad Prism. *P < 0.05, ****P < 0.0001. D: Bright detail similarity (median) measures pixel-by-pixel correlation between TLR4 and EEA1 in each image, effectively measur- ing colocalization of the two probes at each denoted time point. Bright detail similarity >1.0 indicates colocalization of TLR4 and EEA1. *P 5 0.014, **P 5 0.0067. The quantification of C and D represent data collected from three independent experiments.
Ab0006, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 8—TLR4-Ab remains colocalized with EEA1 and sequesters TLR4/MD2 inside the endosome for at least 24 h. BMDMs were pulsed with LPS (100 ng) or TLR4-Ab (UT18 100 ng) for 5 min, washed, and then fixed at the noted time points. Cells were stained (CD11b, TLR4/ UT18, EEA1) for imaging flow cytometry, and 5,000 events (gated on CD11b1 cells) were collected for each cell population. A and B: Rep- resentative images for LPS- and TLR4-Ab–stimulated BMDMs are shown for each time point. C: Internalization analysis generated using IDEAS software provides a mean internalization erode score using CD11b as the surface marker and TLR4/UT18 as the internalization probe. TLR4 internalization scores >1.0 indicate internalization of TLR4. P values calculated in GraphPad Prism. *P < 0.05, ****P < 0.0001. D: Bright detail similarity (median) measures pixel-by-pixel correlation between TLR4 and EEA1 in each image, effectively measur- ing colocalization of the two probes at each denoted time point. Bright detail similarity >1.0 indicates colocalization of TLR4 and EEA1. *P 5 0.014, **P 5 0.0067. The quantification of C and D represent data collected from three independent experiments.

Journal: Diabetes

Article Title: Endosomal Sequestration of TLR4 Antibody Induces Myeloid-Derived Suppressor Cells and Reverses Acute Type 1 Diabetes.

doi: 10.2337/db21-0426

Figure Lengend Snippet: Figure 8—TLR4-Ab remains colocalized with EEA1 and sequesters TLR4/MD2 inside the endosome for at least 24 h. BMDMs were pulsed with LPS (100 ng) or TLR4-Ab (UT18 100 ng) for 5 min, washed, and then fixed at the noted time points. Cells were stained (CD11b, TLR4/ UT18, EEA1) for imaging flow cytometry, and 5,000 events (gated on CD11b1 cells) were collected for each cell population. A and B: Rep- resentative images for LPS- and TLR4-Ab–stimulated BMDMs are shown for each time point. C: Internalization analysis generated using IDEAS software provides a mean internalization erode score using CD11b as the surface marker and TLR4/UT18 as the internalization probe. TLR4 internalization scores >1.0 indicate internalization of TLR4. P values calculated in GraphPad Prism. *P < 0.05, ****P < 0.0001. D: Bright detail similarity (median) measures pixel-by-pixel correlation between TLR4 and EEA1 in each image, effectively measur- ing colocalization of the two probes at each denoted time point. Bright detail similarity >1.0 indicates colocalization of TLR4 and EEA1. *P 5 0.014, **P 5 0.0067. The quantification of C and D represent data collected from three independent experiments.

Article Snippet: BMDMs were plated in 24-well plates at 2 × 106 cells/mL in complete DMEM with Fc block (1:5,000; BioLegend), then pulsed with TLR4-Ab (UT18 100 ng), Ctrl-Ab (UT15 100 ng), or LPS (100 ng) for 5 min. BMDMs were stained in FIX & PERM (Invitrogen) with antibodies against CD11b (BioLegend), EEA1 (Novus Biologicals), and UT18/UT15-specific secondary antibodies (BioLegend).

Techniques: Staining, Imaging, Cytometry, Generated, Software, Marker